Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Tbr1

Cell type

Cell type Class
Neural
Cell type
Cortex
NA
NA

Attributes by original data submitter

Sample

source_name
P2 Wildtype Cortex_TBR1 ChIP-seq
strain
CD1
age
P2
tissue
cortex
chip antibody
TBR1 (Santa Cruz Biotech, Cat# sc-48816 X)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and TBR1-DNA complexes were isolated with antibody. ChIP-seq libraries were generated using Ovation Ultralow System V2 (NuGEN) following manufacturer's protocol. Libraries were sequenced on Illumina HiSeq 4000

Sequencing Platform

instrument_model
Illumina HiSeq 4000

mm10

Number of total reads
38245922
Reads aligned (%)
95.4
Duplicates removed (%)
21.7
Number of peaks
10721 (qval < 1E-05)

mm9

Number of total reads
38245922
Reads aligned (%)
95.4
Duplicates removed (%)
21.8
Number of peaks
10765 (qval < 1E-05)

Base call quality data from DBCLS SRA